Most reconstitution calculators show you the math. This one shows you what you'll actually get. Because a 100-unit vial reconstituted with 2.0ml of saline doesn't always yield 100 units — dead space, adsorption loss, and draw technique mean you may recover only 90-95%. The +10% overfill recommendation is standard clinical practice, and the only way to reliably hit your labeled unit count every time.
OnabotulinumtoxinA · Allergan
Botox
Saline Volume (ml)
Most common: 2.5ml
Choose by Desired Concentration
2.5
mL dilution
4
U per 0.1 mL
40
U per 1 mL
Your Dilution
2.5mL
~92% yield — 4u per 0.1mL
Recommended
With Overfill
2.75mL
+0.25mL overfill — reliably hits 100U
Vials contain labeled units but real-world yield varies due to dead space, adsorption, and draw technique. +0.25mL overfill is standard clinical practice.
Storage: 2–8°CUse within: 24 hours (refrigerated)Source: FDA Prescribing Information
This calculator is for educational purposes. Always follow your local guidelines and manufacturer recommendations.
📋 Botox Notes
Botox — Clinical Reference
Storage
Refrigerate 2-8°C. Use within 24 hours after reconstitution. Do not freeze.
Available Vial Sizes
50U, 100U
FDA-Approved Areas
Glabellar lines, crow's feet, forehead lines
Max Dose
400 units per 3-month interval
Duration
3-4 months typically
Re-treatment
No more frequently than every 3 months
Key Note
Single patient use only. Discard unused portion after 24 hours.
Manufacturer
AbbVie/Allergan (1-800-678-1605)
⚡ Quick Reference
Very dilute (1:1)Great for large areas
Standard (2:1)Most common choice
Concentrated (4:1)Precise, small areas
💡 Pro Tips
- • Always use preservative-free saline
- • Draw up slowly to avoid creating bubbles
- • Reconstituted toxin should be used within 24 hours
- • Store in refrigerator after reconstitution
- • Never shake the vial — gently swirl instead
📊 Why Accurate Dilution Matters
- • Consistent patient results
- • Predictable dosing
- • Reduced waste and cost
- • Better injection control
- • Improved patient satisfaction
Unit Conversion
units of
1:1 Ratio
=20UXeomin
=20UJeuveau
=20ULetybo
1:2 Ratio
=40UDaxxify
1:3 Ratio
=60UDysport
Approximate conversions based on clinical practice ratios (1:1 Botox/Xeomin/Jeuveau/Letybo, 1:2 Botox/Daxxify, 1:3 Botox/Dysport). Units are NOT interchangeable between products. Always follow manufacturer labeling and clinical judgment.